rat-anti-mouse mac2 primary antibody  (Cedarlane)

Journal: Frontiers in Medicine

Article Title: Targeting acid ceramidase ameliorates fibrosis in mouse models of non-alcoholic steatohepatitis

doi: 10.3389/fmed.2022.881848

Figure Lengend Snippet: Male wild-type mice on FPC diet and administered an acid ceramidase inhibitor developed less fibrosis than vehicle control mice. (A) Representative photomicrograph of Sirius red-stained liver sections (top). Sirius red staining was quantified (collagen proportional area, CPA, bottom). (B) Representative immunofluorescence images of mouse liver tissue sections stained with DAPI (blue) and collagen 1 (green) (top). Scale bar: 50 μm. Mean fluorescence intensity (MFI) of collagen type 1 was measured in five sections per mouse ( N = 3 mice per group) (bottom). (C) Representative immunohistochemical images of mouse liver tissue sections stained with Mac2 (top). Scale bar: 50 μm. The number of Mac2 positive cells per 20× microscopic field were measured in five sections per mouse ( N = 3 mice per group) (bottom). (D) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) quantified expression (mean ± SEM) of the indicated genes, compared using one-way ANOVA with Tukey’s method for multiple comparisons. Samples are normalized to Gapdh . * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The liver tissue was incubated with Avidin/Biotin Blocking reagent (Vector Laboratories, #SP-2001) for 15 min and incubated overnight at 4°C with anti-galectin-3 (Mac2) primary antibody (1:100; Santa Cruz, #SC-20157) diluted in 5% normal goat serum.

Techniques: Control, Staining, Immunofluorescence, Fluorescence, Immunohistochemical staining, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Expressing